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rabbit polyclonal antibody against inos  (Bioss)


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    Structured Review

    Bioss rabbit polyclonal antibody against inos
    Rabbit Polyclonal Antibody Against Inos, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+anti+inos/NOS-2+Polyclonal+Antibody/10__1186_slash_s41936___026___00580___8-88-19-24
    Average 94 stars, based on 69 article reviews
    rabbit polyclonal antibody against inos - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Somatostatin prevents lipopolysaccharide-induced neurodegeneration in the rat substantia nigra by inhibiting the activation of microglia
    Article Snippet: The bands of protein separated by SDS-PAGE (8% gel for iNOS, 10% gel for NF-κB p65 and NF-κB p-p65, and 12% gel for Cox-2) were transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA) using an electroblot apparatus (DYCZ-40D, Beijing, China). .. Filters were blocked in 5% non-fat milk and incubated separately overnight at 4°C with the following primary antibodies: Rabbit polyclonal anti-iNOS (1:1,000; cat. no. bs-2072R; Bioss, Beijing, China), goat polyclonal anti-Cox-2 (1:100; sc-1747), mouse monoclonal anti-NF-κB p-p65 (1:100; cat. no. sc-166748) and rabbit polyclonal anti-NF-κB p65 (1:100; cat. no. sc-372) obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). .. Membranes were then washed with Tris-buffered saline containing Tween-20 (TTBS) (BioSharp, Hefei, China) and incubated with the following corresponding HRP-conjugated secondary antibodies: Goat anti-mouse immunglobulin (Ig)G-HRP (1:5,000; cat. no. A0216), goat anti-rabbit IgG-HRP (1:5,000; cat. no. A0208) and donkey anti-goat IgG-HRP (1:5,000; cat. no. A0181) (Beyotime, Shanghai, China) for 45 min at 37°C.

    Article Title: Somatostatin prevents lipopolysaccharide-induced neurodegeneration in the rat substantia nigra by inhibiting the activation of microglia.
    Article Snippet: The bands of protein separated by SDS-PAGE (8% gel for iNOS, 10% gel for NF-κB p65 and NF-κB p-p65, and 12% gel for Cox-2) were transferred onto polyvinylidene difluoride membranes (Millipore, Billerica, MA, USA) using an electroblot apparatus (DYCZ-40D, Beijing, China). .. Filters were blocked in 5% non‐fat milk and incubated separately overnight at 4 ̊C with the following primary antibodies: Rabbit polyclonal anti-iNOS (1:1,000; cat. no. bs-2072R; Bioss, Beijing, China), goat polyclonal anti-Cox-2 (1:100; sc-1747), mouse monoclonal anti-NF-κB p-p65 (1:100; cat. no. sc-166748) and rabbit polyclonal anti-NF-κB p65 (1:100; cat. no. sc-372) obtained from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). .. Membranes were then washed with Tris-buffered saline containing Tween-20 (TTBS) (BioSharp, Hefei, China) and incubated with the following corresponding HRP-conjugated secondary antibodies: Goat anti-mouse immunglobulin (Ig)G-HRP (1:5,000; cat. no. A0216), goat anti-rabbit IgG-HRP (1:5,000; cat. no. A0208) and donkey anti-goat IgG-HRP (1:5,000; cat. no. A0181) (Beyotime, Shanghai, China) for 45 min at 37 ̊C.

    Article Title: Distinct phenotypic expression levels of macrophages in neonatal lungs
    Article Snippet: .. Following addition of 10% (v/v in PBS), the slides were incubated at room temperature for 1 h. The sections were then incubated with rabbit polyclonal anti-iNOS (1:100 dilution; cat. no. bs-2072R; BIOSS) and CD68 (1:100 dilution; cat. no. BSB2717; Bio SB, Inc.,) antibodies at room temperature for 1 h, followed by incubating with polymer double stain detection system (BioTnA), which contained horseradish peroxidase (HRP) green and 3,3'-diaminobenzidine (DAB) brown chromogen, for another 20 min at room temperature. ..

    Polymer:

    Article Title: Distinct phenotypic expression levels of macrophages in neonatal lungs
    Article Snippet: .. Following addition of 10% (v/v in PBS), the slides were incubated at room temperature for 1 h. The sections were then incubated with rabbit polyclonal anti-iNOS (1:100 dilution; cat. no. bs-2072R; BIOSS) and CD68 (1:100 dilution; cat. no. BSB2717; Bio SB, Inc.,) antibodies at room temperature for 1 h, followed by incubating with polymer double stain detection system (BioTnA), which contained horseradish peroxidase (HRP) green and 3,3'-diaminobenzidine (DAB) brown chromogen, for another 20 min at room temperature. ..

    Staining:

    Article Title: Distinct phenotypic expression levels of macrophages in neonatal lungs
    Article Snippet: .. Following addition of 10% (v/v in PBS), the slides were incubated at room temperature for 1 h. The sections were then incubated with rabbit polyclonal anti-iNOS (1:100 dilution; cat. no. bs-2072R; BIOSS) and CD68 (1:100 dilution; cat. no. BSB2717; Bio SB, Inc.,) antibodies at room temperature for 1 h, followed by incubating with polymer double stain detection system (BioTnA), which contained horseradish peroxidase (HRP) green and 3,3'-diaminobenzidine (DAB) brown chromogen, for another 20 min at room temperature. ..



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